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The course will cover in depth knowledge of Forensic Biotechnology in Pharmaceutical Science, it's disciplines and their importance when working in FSL.
Introduction to Recombinant DNA Technology
DNA Modifying enzymes. Cloning strategies: Genomic libraries, cDNA libraries, single gene cloning. RAPD, RFLP and AFLP. Vectors: Types of vectors and choice of vectors : Plasmids, Cosmids, lambda phage, shuttle vectors, bacs and yacs. Transformation and Transfection. Expression system in Eukaryotic cells, Yeast, Bacteria, Insect cell lines, Gene screening. Biosafety guidelines and containment startegies
Bioreactor design: Body construction, aeration and agitation, operation and applications. Microbial growth: measurement, batch and continuous culture and its kinetics. Downstream processing: recovery and purification of products. Strain improvement. Fermentation economics
Cell lines: definition, development, maintenance and management , established cell lines and their characteristic features. Transgenic animals : creating transgenic animals, examples of transgenic animals - Dolly, insects, primates, mice. Somaclonal and gameto clonal variation: applications and limitations. Transgenic Plants: Herbicide resistant, insect Resistant, drought/stress resistant, delayed ripening, Ti Plasmid and T-DNA transfer. Gene therapy: potential approach to gene therapy. Southern blotting, Northern blotting, Western blotting, Iso-electric focusing. Overview of Stem cells and its applications
Introduction to industry important recombinant proteins, recombinant protein stability. Improvised recombinant protein secretion. Introduction to industrially important primary and secondary metabolites from plants and microbes. Microbial production: Antibiotics, acetic acid, lactic acid, wine, beer, SCP
Basic principles of quality control (QC) and quality assurance (QA). Guidelines for QA and QC: raw materials, products and validation. Introduction to pharmacopoeia. Intellectual Property Rights. Importance of protecting scientific discoveries. IPR policy of Government of India. Patent: Qualification (novel, commercial and non-obvious), jurisdiction of patent laws, Indian and international patent laws, filing procedures.
Bernard G. Glick, Jack J. Pasternak and Cheryl L. Patten, ASM Press, ISBN: 978-1555814984
DNA Modifying enzymes mod2 DNADNA Modifying Enzymes
Cloning strategies: Genomic Libraries04_M109nys091016_19_23
cDNA libraries, single gene cloning Send Genomic and cDNA libraries04_M109nys091016_19_23
RAPD, RFLP and AFLP aflp2aflpRandom_amplified_polymorphic_DNA_RAPD_markers_and_18591_Restriction Fragment Length PolymorphismInTech-Restriction_fragment_length_polymorphism_analysis_of_pcr_amplified_fragments_pcr_rflp_and_gel_electrophoresis_valuable_tool_for_genotyping_and_genetic_fingerprintingrflp
Vectors: Types of vectorsMSc-Biotech-02Sem-ProfBhuminathTripathi
Choice of vectors: Plasmids, Cosmid, Lambda Phage vectors119_Cloning vectors
Shuttle vectors, BACS, YACSSend Cloning Vectors-1
Transformation and Transfection transfection-slideshowpeerj-111653 Transformation
Expression system in Eukaryotic cells, Yeast, Bacteriaexpression_systems
Insect cell lines, Gene screeningexpression_systemsIntroduction_to_Genetic_Screening
Biosafety guidelines and containment strategies
Bioreactor body construction. Aeration, agitation. Operation and Principals30042020085526-BIOREACTOR__BASICS-converted_(1)
Microbial growth: Measurement 04_Chapter_33__Microbial_Nutrition_and_Growth_07Chap06Growth1834_indirectmethodsofmeasurementofgrowth
Downstream processing: Recovery and purification of productsSend Downstream Processing-1
Strain ImprovementSend Strain Improvement
Fermentation Economics
Cell lines: Definition, development, maintenance and management, established cell lines and their characteristic features.
Transgenic animals- Creating transgenic animals, Example of transgenic animals-DollySend Transgenic Animals
Insects, Primates, miceSend Transgenic Animals
Somaclonal and gameto clonal variationSend UDAY.SOMACLONAL.
applications and limitations
Transgenic Plants: Herbicide resistant, insect ResistantSend Transgenic Plants
drought/stress resistant, delayed ripeningSend ayaskanta ppt
Ti Plasmid and T-DNA transfer.
Gene therapy: potential approach to gene therapyGene Therapy-1
Southern blotting, Northern blottingSend blotting
Western blotting, Iso-electric focusingSend blotting
Overview of Stem cells and its applicationsstem cells
Introduction to industry important recombinant proteins, recombinant protein stabilitymicrobial recombinant protein
Improvised recombinant protein secretion
Introduction to industrially important primary and secondary metabolites from plants and microbes
Microbial production: Antibiotics, acetic acid, lactic acid Production of Lactic AcidProduction of Acetic Acid
Wine, Beer, SCP Wine ProductionCommercial Production of BeerSingle Cell Proteins
Basic principles of quality control (QC) and quality assurance (QA)_QA-QC
Guidelines for QA and QC: raw materials, products and validation
Introduction to pharmacopoeia000214416
Intellectual Property RightsINTELLECTUAL-PROPERTY-RIGHTS_20.04.2020
Importance of protecting scientific discoveries
IPR policy of Government of IndiaIntellectual Property Right (IPR) and Protection(IPP
Patent: Qualification (novel, commercial and non-obvious), jurisdiction of patent laws, Indian and international patent laws, filing procedures.IPR